If you are new to DSI Studio, I would recommend to start with Read DICOM, NIFTI, Bruker 2dseq, or Varian FDF files. This gives you a step-by-step manual to create an SRC file from DICOM files or raw data. Once you get the SRC file, you may continue to do the reconstruction. An SRC file can be reconstructed by DTI, GQI, or QSDR. All these methods can be applied to conventional DTI dataset. The reconstruction generates an FIB file, which allows you to do fiber tracking. You may need to familiarize with the details about how to use ROIs and tune the fiber tracking parameters. Until here you already have the basic skill to generate fiber tracks, and you can move on to the topics about How to analyze diffusion data? This includes basic methods to more advanced approaches. You may also need to know how to visualize your result (see the Visualization section below) or use command line interface to process a large amount of data.
From Diffusion Data to Tractography
Connectometry and ConnectomeFingerprint