Academic License:
     DSI Studio is free for research purposes under the Attribution-NonCommercial-ShareAlike 2.0 Generic (CC BY-NC-SA 2.0) licensePlease note that the license provides no warranties. 

Clinical License:

    *The sole purpose of this clinical license is to minimize the liability of clinical users due to software issues. You may still opt to choose the academic license and assume the responsibilities to actively update DSI Studio in time for bugs and software errors.

    DSI Studio is free for clinical use. Users under a clinical license will receive notifications of critical updates that may affect the tractography quality. The bug report and feature suggestions from clinical license users will be placed on high priority. The license follows CC BY-NC-SA 2.0 license with the following additional requirements: 

     1. The license is for one year and can be renewed every year.
     2. Users must maintain a valid IRB in the licensing period.
     To obtain this license, please fills out the following form and provide the IRB number: The license will be provided through an email notification. 

Commercial License:

     The DSI Studio source code is free for commercial usage under GNU General Public License
     The figures derived from DSI Studio can be freely used and distributed under CC BY-SA 4.0 license if attributing the contribution of "DSI Studio at"
     Other commercial licenses can be provided after contacting Frank Yeh at

Download link:

     *You may need to install Microsoft redistributable package here to run DSI Studio if it complains missing some dll files.
     Please download the zip file from one of the following links and unzip it to a folder. Click on the DSI Studio program icon to run it. 
     Google Drive
     One Drive

     MAC OS X 
     Please download dsi_studio_64.dmg from one of the following links and open it. You may need to enable the permission to run a 3-party app (see instruction).

     *IMPORTANT* DSI Studio will crash on Mac OS 10.12 Sierra due to incompatibility problems in the OpenGL functions (no solution identified yet). Please upgrade Mac OS to the most recent version, or you may need to use an older version of DSI Studio before 2019 (link to the previous versions provided in the bottom). I am sorry for the hassle.

     Google Drive
     One Drive

     docker image: docker://dsistudio/dsistudio
docker run -ti --rm -e DISPLAY=$DISPLAY \
                    -v /tmp/.X11-unix:/tmp/.X11-unix \
                    -v $PWD:/data \

$ module load singularity
singularity pull docker://dsistudio/dsistudio     
singularity exec dsistudio_latest.sif dsi_studio   #GUI
singularity exec dsistudio_latest.sif dsi_studio --action=rec --source=my.src.gz --thread_count=4 --method=7 --param0=1.7  #command line

     Compile instruction available here. Please make sure to download the binary package from and set the default path to QT for version 5.12 and not any other version.

     Previous versions can be found here.

Optional Package:

     Neonate/Infant1yr/Infant2yr template: Download 
     To install, unzip the files and move them to the /template folder (Windows). For Mac users, right click on to [Show Content] and move the files under Content/MacOS/template

Source Code:


     DSI Studio has a very high turning rate, and occasionally the computation outcome may be different between versions. I would recommend users keep a local copy of DSI Studio for each research project and only update DSI Studio if a new project is initiated.

Hardware recommendation:

     You can use regular laptops or workstations to run DSI Studio. To achieve the best performance, I would recommend a computer with multi-core CPU (e.g. A minimum memory of 2GB is needed. To handle a large number of tracks or image volumes, DSI Studio will need 32GB of memory or more. 

     GPU computation is not yet supported in DSI Studio. 

Recent Updates:

     02/28/2019 DSI Studio now supports automatic fiber tracking. 

     06/11/2018 DSI Studio now is able to show graph presentation. Check out:

     06/08/2018 New network measures implemented: Rich club with level=5, 10, 15, 20

     05/07/2018 Calculate helix angle for cardiac DTI
     05/02/2018 QSDR reconstructed FIB will also output DTI measures (FA, ADC, MD)

     04/11/2018 DIS Studio can do distortion correction similar to TOPUP but will use the entire DWI. It will need full AP or PA (LR or RL also works). Check out:

     04/04/2018 User can use a simple "parameter ID" to run command line fiber tracking. There is no need to assign each of the tracking parameters.

     03/05/2018 Connectometry can now be applied to animal scans.

     03/01/2018 QSDR supports any external templates. It can be applied to animal scans.

Registration (OPTIONAL)

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