Nature Methods 2025Platform paper →

Tractography, connectomics and fiber data, in one open platform.

Reconstruct diffusion MRI, map white-matter pathways, run group analysis and segment brain MRI.

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Sun 2026.7.25 · Windows · macOS · Linux · Docker · source on GitHub

Left arcuate fasciculus tractography — Left arcuate fasciculus, DSI Studio rendering

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Start from processed .fz / .sz / .dz files instead of raw DWI.

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AI agents, in the Sun version

Connect a supported local agent and ask in plain language: it reads the manuals, inspects the current data and runs reconstruction, tracking, image processing or connectometry.

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youTrack the left arcuate fasciculus in this FIB file and export FA along the tract.

agentOpened subject01.fz · AutoTrack AF_L · FA along tract saved (illustrative)

rundsi_studio --action=atk --source=subject01.fz --track_id=AF_L

Nature Reviews Neurology covers featuring DSI Studio tractography

Research

DSI Studio has been cited in Nature Neuroscience, Nature Human Behaviour, Brain and NeuroImage, and its tractography appeared on the cover of Nature Reviews Neurology (2017).

DTI · GQI · QSDRAutoTrackDifferentialCorrelationalU-Net segmentation

Nature Methods · July 2025 Yeh, F.-C. DSI Studio: An integrated tractography platform and Fiber Data Hub for accelerating brain research. doi:10.1038/s41592-025-02762-8 · How to cite

Fiber Data Hub

Compact processed diffusion MRI derivatives (.fz, .sz, .dz) that open directly in DSI Studio, without repeating preprocessing from raw DWI.

Kidney rainbow — Ex-vivo and histology

High-resolution ex-vivo diffusion MRI and MRI microscopy, for structural analysis of biological tissue.
Kidney Rainbow by Nian Wang, Center for In Vivo Microscopy, Duke University. Coverage by Science.

U-Net segmentation of mouse, marmoset and rhesus MRI — Brain MRI segmentation

U-Net based segmentation for structural MRI runs inside DSI Studio.
Segmentation examples from U-Net Studio, the sister project.

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